Milliseconds photon-to-photon latency projection system for adaptive optogenetics applications

Area of Science:

• Biophysics
• Optical Engineering
• Microscopy

Background:

• Dynamic projection mapping is essential for adapting projections to moving objects.
• Optogenetics requires precise light stimulation of photosensitive cells, which can be challenging with moving or deforming subjects.

Purpose of the Study:

• To develop a projection method for microscopy with millisecond latency.
• To enable dynamic projection mapping for optogenetics on moving cells.

Main Methods:

• Utilized a low-latency digital micromirror device.
• Integrated a high-speed vision system for real-time tracking.
• Developed a projection system responding to object movement and deformation.

Main Results:

• Achieved a photon-to-photon latency of 6.56 ± 1.76 ms.
• Successfully demonstrated dynamic projection mapping on a randomly moving target.
• Validated the system's capability to respond to object deformation and movement.

Conclusions:

• The proposed method provides a viable solution for dynamic optogenetic stimulation in microscopy.
• This technology opens new possibilities for precise manipulation of biological samples in real-time.