Application of Organoids to Investigate Virus-Host Interactions
Tong Wu1, Quan Zhou1, Chunfu Zheng2
1Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong Province, China.
Related Experiment Videos
View abstract on PubMed
Summary
Organoids, or 3D stem cell-derived tissues, offer advanced models for studying complex virus-host interactions. This chapter details their use in understanding viral dynamics and provides experimental protocols.
Area of Science:
- Virology
- Stem Cell Biology
- Tissue Engineering
Background:
- Traditional models struggle to capture complex virus-host interactions.
- Organoids provide a more physiologically relevant 3D system for studying human tissues.
- Understanding virus-host dynamics is crucial for developing antiviral strategies.
Purpose of the Study:
- To summarize the applications of organoids in virus-host interaction studies.
- To provide experimental procedures for utilizing organoids in virology research.
- To highlight organoids as transformative tools in infectious disease modeling.
Main Methods:
- Utilizing stem cell-derived three-dimensional (3D) organoid models.
- Culturing and maintaining organoids for experimental infection.
- Analyzing virus-host dynamics within the organoid microenvironment.
Main Results:
- Organoids successfully model complex virus-host interactions.
- 3D tissue structures reveal intricate dynamics not seen in traditional models.
- Experimental procedures enable detailed investigation of viral pathogenesis.
Conclusions:
- Organoids are powerful tools for advancing our understanding of virus-host interactions.
- The application of organoids facilitates the development of novel antiviral therapies.
- Organoid technology represents a significant advancement in infectious disease research.